Sentence examples for blocking buffer according to from inspiring English sources
Endogenous peroxidase was blocked using the DakoCytomation Envision System-HRP blocking buffer according to the manufacturer's protocol (DakoCytomation, Carpinteria, CA).
Then, cells were fixed and blocked with 1X blocking buffer according to the manufacture's protocol.
Subsequently, tissue was treated with blocking buffer according to the manufacturer's instruction (Perkin Elmer, Rodgau, Germany).
The tissue was blocked using 5% goat serum 4% BSA blocking buffer according to standard protocol.
Antibodies were diluted into fresh blocking buffer according to the manufacturer's recommendations, and the membranes processed as described [ 59].
Total protein was extracted using radioimmune precipitation buffer protein lysis buffer according to protocols (Beyotime, Shanghai, China).
Sample plus control labeled DNAs were mixed with human Cot-I DNA for blocking unspecific hybridization and mixed in blocking and hybridization buffers according to the manufacturer's protocol.
The probe was detected using anti-DIG-alkaline phosphatase, blocking, wash and detection buffers according to the manufacturers instructions (Roche).
In combination with FastRed in situ detection using anti-DIG-AP Fab fragments (Roche Diagnostics) at 1 500, primary antibodies were added to blocking buffer together and incubated according to the in situ protocol, and secondary antibody detection was carried out after the FastRed staining was completed and following three 1X PBT washes.
Before analysis, plasma and blood cell samples were pretreated using dilution in a formulated saline buffer with 1% blocking polymer according to the supplier's instructions.
DIG Wash and Block Buffer Set (Roche) was used for washing and blocking incubations according to manufacturer's instructions, and detection was made with CDP- Star (Roche) also following the instructions.
