Sentence examples for blocking agent was added from inspiring English sources
A serial dilution of serum in PBS with 10% blocking agent was added to the wells and the plates incubated for 2 h at 37°C.
After blocking with 5%% skim milk at 37 °C for 1 h, 50 μl of the serum sample 1 20 diluted in blocking agents was added to each well, followed immediately by the addition of 50 μl of MAb 3 F9 supernatants 1 400 diluted in blocking agents.
The early finding that attracted the most attention and controversy was presented roughly 60 years ago when a six-fold higher mortality rate when neuromuscular blocking agents were added to anesthetics was reported.
Briefly, a scratch was made with a sterile 100 μl pipette tip in a confluent cell layer, washed twice in physiologic saline, and then various stimulatory or blocking agents were added in serum-free medium.
No neuromuscular blocking agent was administered in this study.
No additional neuromuscular blocking agent was given during surgery.
It was detected that the addition of 3 mg/kg paracetamol and 50 mg dexketoprofen into local anesthetic agents as adjuvant in regional intravenous anesthesia performed for hand and/or forearm surgery reduced VAS values and shortened sensorial block onset time and motor block return time significantly when compared with patients to whom no adjuvant agent was added.
After cooling, 2 ml of methylation agent was added.
Similar results were obtained when a stabilizing agent was added.
No activating agent was added to the PRP.
No muscular blocking agents were used in this study.
