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The depletion of FLNa by siRNA blocked the internalization of CCR2B even at 30 min after ligand stimulation (compare Figure 8 Ai to Aii).
To examine the role of activity-dependent endocytosis of A-type channels in intrinsic plasticity, we blocked the internalization of A-type K+ channels by including a dynamin-based peptide (DYN, 100 µg/ml) in the patch pipette before applying paired conditioning stimulation (Figure 5 and 6).
Dyngo treatment blocked the internalization of TfR.
Furthermore, the Cat1-a mutation notably blocked the internalization of Cat1-EGFP by the cycloheximide treatment, similar to that observed in arn1Δ (Fig. 3E, Fig. 8D).
Meanwhile, the preferential effect of Nef on M2-M Φ correlated with the fact the Nef entered M2-M Φ more efficiently than M1-M Φ. Importantly, the macropinosome formation inhibitor EIPA completely blocked the internalization of Nef into M2-M Φ.
Previous studies have shown that treating host cells with the β subunit of cholera toxin, which is produced by the bacterium Vibrio cholera and binds to the GM1 ganglioside, a well-known marker of lipid rafts [ 17], markedly blocked the internalization of T. cruzi by macrophages [ 19].
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This was further proven by the results that the inhibition of phagocytosis did not block the internalization of QDs into protozoans.
We have now shown that blocking the internalization of the CysLT1R does not reduce this phosphorylation but, instead, a slight increase of the signal is detected (Fig. 9A).
The antibody thus appears to block the internalization of exogenous ATXN1 oligomers rather than interacting directly with intracellular oligomeric species.
Similarly, treatment of cells with Dyngo seemed to completely block the internalization of endogenous ABCB6, as ABCB6 remained on the surface throughout the entire 30-min time course.
In contrast, Arf6 Q67L overexpression was found to completely block the internalization of MHC class I molecules ([11] and data not shown).
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