Sentence examples for blocked membrane from inspiring English sources

A similar phenomenon was reported for the R-SNARE gene SEC22 in Arabidopsis mutants (El-Kasmi et al. 2011), which show fragmentation of Golgi stacks, presumably because of blocked membrane fusion during anterograde or retrograde ER to Golgi trafficking (El-Kasmi et al. 2011).

Deleting F313 and F314 presumably blocked membrane insertion and/or the stability of the pore in the membrane.

After washing 4 times (10 min each) with TBST, the blocked membrane was incubated in a sealed bag with 1∶1000 diluted monoclonal antibody to σ32 (Neoclone) at 4°C overnight.

CD83 was detected by incubating the blocked membrane with a 1∶10.000 fold dilution of the polyclonal rabbit anti-mouse CD83 serum, followed by incubation with a 1∶2000 dilution of HRP conjugated goat anti-rabbit immunoglobulin (Dako, Glostrup, Denmark) and developed with ECL™ Western Blotting Detection Reagents (Amersham Biosciences, Buckinghamshire, England).

The blocked membrane was then incubated with various antibodies.

The blocked membrane was incubated overnight in blocking buffer containing primary antibody, followed by five washes.

Blocked membranes were incubated sequentially with primary and secondary antibodies (Supplementary Table S1) and washed with Tris-Buffered Saline with 0.1% Tween 20 (TBST).

Blocked membranes were incubated with either AMPK α (1 1,000), phosphorylated-AMPK α (Thr 172) (1 500), or insulin receptor β (1 1,000) (Cell Signaling Technology, Danvers, MA, USA).

Blocked membranes were incubated with appropriate primary antibodies for 1 h at room temperature.

Blocked membranes were reacted with anti-hPaf1 mouse monoclonal antibody at 1 µg/ml in PBS containing 0.05% Tween-20 (PBST) overnight at 4°C.

Blocked membranes were sealed in a bag with 125 μl/sq.