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Since we reasoned that embryonic toxicity of high shRNA expression may be the cause of this failure, we decided to use constructs with an H1 promoter blocked by the insertion of an operator (tetO) controlled by the tetracycline-repressor (tetR) and the simultaneous and ubiquitous expression of a codon-optimized tetR.
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Furthermore, it was found that the primary styrene insertion gives rise to complexes in which the active sites are blocked by the phenyl ring in both metal atoms, so that only the secondary insertion of the styrene is possible.
This, too, was blocked by the Justice Department.
All malware was blocked by the firewall.
The channels of membrane based heat exchanger are partially blocked by insertion of metal blocks.
In StPurL, this cavity entrance is blocked by a loop insertion of residues 668−677, which interacts with helix α24 (α17 of TmPurL), and further blocked off by the glutaminase domain, making the nucleotide structural in StPurL and likely inserted during protein folding.
Interestingly, in C. alai this block is interrupted by the insertion of another conserved block consisting of trnS, trnL, and NAD4 which corresponds to C. albicans TU8.
The exhaust pipe was blocked by snow.
The charge of the blocked ions is compensated during redox-switching by the insertion of the small cations into the polymer film and the screened ions do not participate in redox process.
The synteny is disrupted after the second RHS gene by the insertion of a block containing RNA helicase, N-acetyltransferase and hypothetical protein genes.
Patients that have received bone grafts (particulate or in block) during the insertion surgery of the dental implants; 6.
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