Moreover, adipogenic differentiation could be blocked by the addition of autophagy inhibitors.
The interference could be blocked by the addition of rat immunoglobulin to the sample buffer.
It was previously demonstrated that uptake of [11C]erlotinib in HCC827 xenografts could be blocked by the addition of unlabeled erlotinib [29].
Indomethacin (1 μmol/L) completely inhibited PGE2 production but augmented collagen degradation (17.9 vs 2.3 μg per gel), and this was blocked by the addition of exogenous PGE2.
Result show that 20 ng/mL or 100 ng/mL NGF could significantly promote the proliferation of ORS cells in vitro, but the promotion could be blocked by the addition of 20 ng/mL K252a, the inhibitor of TrkA.
The channels seem to mainly allow calcium ions to pass since gravitaxis is blocked by the addition of the calcium ionophore A23187 and by vanadate which blocks the Ca-ATPase in the cytoplasmic membrane.
Endogenous alkaline phosphatase was blocked by the addition of 2 mM levamisole.
Interestingly, these responses are blocked by the addition of serine-protease inhibitors to the CF, indicating serine-protease dependency.
The effect of LCT was blocked by the addition of an inhibitor of chylomicron secretion, Pluronic L-81.
The stimulation of megalin mRNA expression was blocked by the addition of specific PPARα or PPARγ antagonists.
Similar to the block of PKA activity by PKI, phosphorylation of CREB by IBMX or forskolin was also blocked by the addition of PKI (Fig. 4E).
