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In order to test the specificity of the uptake of 10 by either PC3-PIP tumor (PSMA mediated) or U87-MG tumor (αvβ3 integrin mediated), a blockade experiment was performed after developing an in vivo system that harbors all three tumor types concurrently (group-3).
In order to investigate further the gating-dependence of IHERG blockade by doxepin, experiments were performed using two inactivation-attenuating HERG mutants: S631A and N588K.
These experiments were performed with a number of acute interventions affecting the proposed pathway, including vagotomy, carotid body denervation, and TRPV1 and/or LPA receptor blockade.
All experiments were performed once.
Experiments were performed as described1.
Four independent experiments were performed.
Experiments were performed in triplicate.
Three independent experiments were performed.
Experiments were performed by UCL Genomics.
Two complementary experiments were performed.
Experiments were performed at 25 °C.
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