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Half of the plots within each block were treated with 0.081 ml/mg dry matter (DM) of a solution containing 110 g lactic acid/kg and non-viable Lactobacillus acidophilus at the time of mowing (T) and half were not treated (U).
Two branches from each block were treated with: 6000 × Flusilazole + 300 × PBO (calyx abscising treatment) or GA3 50 mg.L-1 (calyx persisting treatment) sprayed at 0 d after full bloom, with plants with no treatment as the control.
Four trees from each block were treated with: 1) Control (water); 2) NAA (Fruitone N; AMVAC, Newport Beach, CA) at 15 mg L-1; 3) Shading (92% polypropylene shade over the entire tree for five consecutive days and then removed), respectively.
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The experiment was set up as a block design and each block was treated with different fertilizer strategies in triplicate: no fertilizer (CK), inorganic phosphorus (P) and potassium (K) fertilizer (PK), inorganic N, P and K fertilizer (NPK), and inorganic P and K fertilizer plus manure (MPK).
For IHC of the p53 protein expression in the tumour tissue, the paraffin-embedded tissue block was treated with 0.3% H2O2 in methanol to block endogenous peroxidase, and heated in a microwave oven for 20 min for antigen retrieval.
In our previous study, wood blocks were treated with a single particle size (30 nm) of ZnO and the effect of particle size could not be ascertained.
In control cultures, tissue blocks were treated with culture medium only.
Beech and pine wood blocks were treated with 1,3-dimethylol-4,5-dihydroxyethylen 1,3-dimethylol-4,5-dihydroxyethylen 1,3-dimethylol-4,5-dihydroxyethylen 1,3-dimethylol-4,5-dihydroxyethylen
The blocks were treated with 1% OsO4, dehydrated with a graded series of ethanol and propylene oxide, and embedded in epoxy resin.
Tissue cores (1.5 mm) extracted from the source blocks were treated with xylene and ethanol, as described by Garnis et al. [ 11].
Six to ten enamel blocks were treated with the same chemotherapeutic and subsequent to treatment, half of the blocks were directly measured for surface free energy changes, while the other half of the blocks were exposed again to reconstituted saliva for 3 h, prior to surface free energy assessment.
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