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Three 20 μm tissue slices from each tissue block were transferred to a sterile 1.5 ml vial. 1 ml of xylol was added and incubated for 5 minutes.
Four 8-mm-thick cryosections from each block were transferred to nitrocellulose membranes that had been dampened with lysis buffer (0.5% NP-40, 0.5% sodium deoxycholate, 100 mM NaCl, 10 mM EDTA, 100 mM Tris HCl pH 8.0).
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Each random permuted block was transferred to a sequence of consecutively numbered, sealed, opaque envelopes and these were stored in a locked drawer until required.
The tissue blocks were transferred to a recipient "master" block using a Tissue Microarrayer.
Twenty-four hours after seeding, the small tissue blocks were transferred to new culture plates.
Next, the epoxy blocks were transferred to a microgrinding machine, polished, and stained with toluidine blue.
Fresh 2 μm sections from TMA blocks were transferred to glass slides, dewaxed and rehydrated.
These blocks were transferred to fresh PBS-glucose-MgCl2 and the SVZDL and dorsal cerebral cortex were grossly isolated.
Four μm sections of the resulting TMA blocks were transferred to an adhesive coated slide system (Instrumedics Inc., Hackensack, NJ).
Two sections of the TMA blocks were transferred to an adhesive coated slide system (Instrumedics Inc., Hackensack, NJ, USA).
For AFM studies, the tumor sections in paraffin-embedded blocks were transferred to poly-L-lysine-coated glass slides and air-dried overnight at 37°C.
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