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Three cores from each tumour block were sampled with 1.0 mm punchers with a manual tissue microarrayer (Tissue Arrayer 1, Beecher Instruments Inc., Silver Spring, MD, USA).
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Paraffin-embedded donor tissue blocks were sampled with 0.6 punchers using a tissue microarray (TMA) instrument (Beecher Instruments Inc., Sun Prairie, WI, USA).
In a first step, 40 of the 400 enumeration areas- EAs (clusters) provided by the 2002 census blocks were sampled randomly with probability proportionate to size, the size being the number of household in the cluster.
Secondly, the computational cost is kept low because locally the optimal label of each individual block is sampled from a small space with its cardinality ≤8 because there are at most 8 members in the voting pool.
The sample blocks were cut with a diamond knife (Diatome, Bienne, Switzerland) on a Reichert-Nissei Ultracut microtome (Leica AG, Vienna, Austria) to obtain ultrathin sections (90 nm), which were collected on copper grids (200 mesh).
Shown are samples blocked with N-ethyl maleimide at the time of lysis and resolved by reducing and non-reducing SDS-PAGE.
Tissue microarray blocks were constructed with the duplicated test sample and 12 controls.
A map of the receiver blocks was prepared with coordinates for each sample to correctly identify the tumour samples.
As the basement rocks in each geological block are generally considered as the source of younger detrital sediments in the block, each sample is categorized with respect to the geological block(s) exposed in its drainage basin (Fig. 8).
Then, the tephra-bearing ice block samples were measured with a three-axis LTS-SQUID gradiometer developed for nondestructive evaluation.
Because the gray pumice and lava block samples were charged with microlites in the matrix, we did not use micro-FTIR.
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