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WASHINGTON — The tables, which stretched the length of a full city block, were covered with pork lettuce wraps, catfish, spring rolls, all manner of vegetable soup and something called lunchtime waffles.
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The tissue block was covered with OCT embedding medium and rapidly frozen at −40°C in 2-methylbutane.
The plastic block was covered with a 3.81-cm-thick green hunting seat cushion (nitrile rubber/PVC foam) to prevent the mice from injury if they fell from the pole.
Any visible parts of the wooden blocks were covered with soil so that only the immobilized crickets could be observed.
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None of the FFB onlay blocks was covered with membranes.
Some were covered with blocks of ice to fend off the summer heat, others were wrapped in white sheets and lined up in rows so family members could identify the victims.
Subsequently, the membranes were covered with blocking solution for one hour at room temperature with gentle rocking before being rinsed two more times for 5 minutes in TBST.
Slides were covered with Peroxidase Block (Merck, Germany) for 5 min to quench endogenous peroxidase activity, followed by incubation with 10% of rabbit serum in 50 mM Tris-HCl, pH 7.4, for 30 min to block non-specific binding sites.
After treatment with protein block serum at room temperature, sections were covered with primary antibodies and incubated at 4°C overnight.
The tubes for folate analysis were covered with foil to block light.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com