Sentence examples for block were applied for from inspiring English sources

To investigate dose-dependency of the effects, four degrees of block were applied for each current.

Serum block was applied for 30 min at room temperature before incubation of the primary antibody.

Primary antibodies were used according to the manufacturer's recommendations (1 : 100), and peroxidase block was applied for 10 min.

Endogenous peroxidase was blocked using Dako peroxidase block for 5 min and then Dako protein block was applied for 5 min after washing in tap water.

Three haemaxytolin-eosin-stained sections (5  μm thick) from a representative paraffin-embedded block were applied on non-cover-slipped slides for microdissection and DNA extraction.

Phalloidin-Alexa Fluor 568 (Invitrogen A12380, diluted 1 50 in blocking buffer) and/or BrdU-FITC (Becton-Dickinson 347583, diluted 1 4 in blocking buffer) were applied for 30 min before final washes and mounting in Vector-shield containing 0.5 mg/ml DAPI (Sigma D9542).

After extensive wash the secondary antibodies (in blocking buffer) were applied for 1.5 hours at RT in darkness.

Either rat anti-mouse CD31 or isotypic antibodies 1∶100 in 10× diluted blocking buffer were applied for 1.5 h and then sections were washed with PBS.

Secondary antibodies in blocking solution were applied for 1 h at room temperature (RT).

Then, rhodamine-conjugated anti-rabbit IgG and FITC-conjugated anti-mouse IgG polyclonal antibodies (each 1 500 in blocking solution) were applied for 30 min, and the slides were observed using a fluorescence microscope (Leica Microsystems).

After washing, the respective secondary antibodies (goat anti-rat 488, goat anti-mouse 568; goat anti-mouse 488, goat anti-rabbit 568, all Molecular Probes (Eugene, OR, USA), 1/1000 in blocking solution) were applied for 2 h at room temperature.