The parsimony tree file for each 4gamete block was analyzed by the program TreeScan [27], which identifies the edges in the tree and, for each edge, the alleles that belong to the two clades defined by the edge.
This was repeated for each of the three blocks and the sample for each block was analyzed by the Cornell Nutrition Analysis Laboratory (Ithaca, NY).
The formed haplotype blocks were analyzed by using the PLINK software, with the sliding window approach, regression models, and controlling for age and sex.
The amplitudes of raw MEPs recorded during baseline blocks were analyzed by means of a mixed-model ANOVA, with Muscle (FDI and ADM), TMS Intensity (120% and 140% of rMT) and Session (PRE and POST) as within-subjects factors, and with Experiment (arm forwards, EXP1, and arm backwards, EXP2) as a between-subjects factor.
Tissue cores (3 mm diameter; 0.8 to 1.7 mg) punched from the formalin-fixed, paraffin embedded tissue blocks were analyzed by mass spectrometry (MS) [ 26].
Paraffin-embedded tumor blocks were analyzed by immunohistochemistry for DCC and p53 proteins expression using a standard avidin-biotynilated peroxidase complex (ABC) staining method [ 15].
The significance of each haplotype within the defined blocks was analyzed by PLINK software [ 25] (http://pngu.mgh.harvard.edu/~purcell/plink/).edu/~purcell/plink/
The synthesized block copolymers were analyzed by 1H NMR spectroscopy on a Bruker (Avance DPX) spectrometer working at 400 MHz.
Linkage disequilibrium (LD), haplotype blocks and haplotype frequencies were analyzed by Haploview (version 4.2) (Gabriel et al. 2002).
Structural heterogeneities in commercial polymers such as poly vinyl chloride) (PVC), segmented copoly etherester) block copolymer, and nitrile rubber were analyzed by cross-polarisation/magic angle spinning (CP/MAS) 13C nuclear magnetic resonance (n.m.r).
