Sentence examples for block was washed with from inspiring English sources

The block was washed with RNase Zap (Ambion, Life Technologies, Grand Island, NY) between samples.

The blocks were washed with 1 mL of 50 mM EDTA (three times) and were then loaded into wells made with the 1% Certified Megabase agarose gel (Bio-Rad Laboratories, HerCAles, CA, USA) in 0.5 × TBE (25 mM Tris, 25 mM boric acid and 20 mM EDTA).

The fixed tissue blocks were washed with 0.1 M Na cacodylate – HCl buffer pH 7.4 (3 × 15 min).

Blocking membrane was washed with 0.1% PBST and probed with primary antibodies (actin, SOD1, and Hsp70).

Blocking reagent was washed with PBS twice for 5 min each and primary antibody was added (bcl2, caspase 3 and 8).

Following blocking, the membrane was washed with 1x PBS containing 0.1% Tween-20 (wash buffer) and incubated with either 6xHis mAb (Clontech) at 1∶1000 dilution [Fig. 1d, e] or IIC5B10 mAb (MR4) at 1∶5000 dilution [Fig. 1f] for 1 h at 22°C.

After each block of stimuli, the syringe was washed with hexane (Merck, Darmstadt, Germany), heated up to 48 °C, and rinsed with continuous clean air for 6min.

The blocked plates were washed with PBS-T four times and the pre-incubated sample was transferred to the freshly washed plates followed by further incubation at room temperature for 2 h.

Blocking was discarded and wells were washed with PBST and incubated with serial dilutions of IgYs in PBST containing 0.2% BSA (100 μl/well, 2 h at 37°C).

The fixative was removed, and the brains were washed with blocking solution (1× PBS with 0.3% TritonX and 0.1% BSA).

The membranes were washed with blocking buffer without milk, and then incubated with horseradish peroxidase-conjugated secondary antibody.