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Paraffin block was sectioned using a rotary ultra-microtome, distributed onto glass slides and then dried overnight.
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Resin was cured at 60°C for 24 h, and blocks were sectioned using a Reichert Ultramicrotome.
EPON blocks were sectioned using an ultramicrotome (Ultracut E, Reichert-Jung and Leica).
Blocks were sectioned using either a Shandon or Leica Cryostat into 12 μm thick sections in a sagittal orientation.
The OCT blocks were sectioned using a Microm HM 550 Cryostat (Microm International GmbH, Walldorf, Germany) into 14-μm thin sections that were placed on SuperFrost Plus slides (Menzel-Gläser, Braunschweig, Germany) and stored at −20°C until use.
The embedded cells in blocks were sectioned using a diamond knife on a Leica Ultracut R microtome and ultrathin sections of 90 nm were collected onto pioloform-coated 100 mesh hexagonal copper grids.
The blocks were sectioned using a diamond knife (Diatome) with an ultramicrotome (UC7, Leica) at a nominal thickness of 70 nm, and the sections were collected on 200 mesh nickel grids dried and observed in a STEM (HD2300-A, Hitachi) with an acceleration voltage of 80 kV.
The block containing the tissue was sectioned using a cooled (-20°C) cryostat (Microm Cryo-Star HM 560, Kalamazoo MI) at 15 μm thicknesses.
The paraffin blocks were sectioned longitudinally using a microtome to give 5 μm sections.
Tissues blocks were sectioned at −16°C using a cryostat (CM 3050; Leica, Germany) to a thickness of 5 µm, as described in previous reports [44].
Tissue blocks were sectioned at −16 °C using a cryostat (CM 3050; Leica, Wetzlar, Germany) to a thickness of 5 μm as previously described.
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