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The TMA recipient block was sectioned on a routine microtome machine.
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Tissue blocks were sectioned on a Leica CM3050 S cryostat.
Methacrylate blocks were sectioned on an MT-990 microtome (Boeckeler Instruments, Tucson, AZ, USA).
Blocks were sectioned on a Leica ultramicrotome, placed on grids and examined in a Phillips H-100 electron microscope.
Embedded blocks were sectioned on a Struers Accutom-50 precision cut-off saw (Struers, Copenhagen, Denmark) using a R330 diamond wafered wheel under water irrigation.
Each block was sectioned at one to three levels, depending on the size of the tissue in the block, and stained with haematoxylin and eosin (H&E).
Paraffin blocks were sectioned to 4 μm thickness and placed on positively charged glass slides.
TMA blocks were sectioned to produce 4-µm sections.
Proximal tibia specimens were cut into two pieces in the sagittal plane, proximal femoral specimens were cut into two pieces in the coronal plane, and specimens were embedded in paraffin blocks to be sectioned on their cut surfaces.
Tissue blocks were sectioned, mounted on glass slides, deparaffinised, and stained with haematoxylin and eosin.
Paraffin-embedded blocks were sectioned, mounted on Superfrost plus slides, de-waxed, and then brought to water.
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