The brain blocks were mounted in O.C.T compound (Miles Inc., Elkhart, IN), rapidly frozen in a dry ice/acetone bath, and then stored at −65°C until dissection.
Inside the MASC, this copper cooling block was mounted to a small flange in the chamber door.
The brains were removed and post-fixed (24 hr, 4°C) in 4% PFA in PBS, incubated in 30% sucrose (12 hr), and the blocked brain was mounted in cryo-embedding media (OCT, Ted Pella, Redding, CA, United States) on a cryostat for sectioning.
Each TMA block included 98 tissue cores and the total RCC material was mounted in six blocks.
The probe tip was mounted in a metal block to exclude ambient light, and the probe illuminated a measurement area of 3 mm on the feather sample.
The detector was mounted in a holder and positioned immediately above the surface of the stack of gel blocks.
In addition to this, Hall probe measurements are performed for magnet blocks while they are mounted in a specially designed cassette with vanadium-permendur poles.
After washing (3×20 min) in blocking buffer, sections were mounted in Mowiol (Calbiochem -containing 4% DABCalbiochem -containing
The eight keys of nickel silver are mounted in blocks.
The frozen tissue blocks for localisation of AlPcS2a were mounted in Tissue Tek II embedding compound (BDH, Poole, UK), and 8 μm sections were cut with a cryostat microtome and mounted on clean glass slides.
