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Each peri-implant bone block was fixed in buffered formalin solution, pH 7.0, for 6 days, demineralized in 10% formic acid, and dehydrated through progressing alcohol concentrations and paraffin-embedded.
In the second, third and fourth stimulus presentation blocks, orientation still varied randomly (blockwise randomization), but the contrast within each block was fixed: in the second block to low contrast only, in the third block to medium contrast only, and in the fourth block to high contrast only (Fig. 1A, "low", "medium" and "high contrast").
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Cryosections of soleus and EDL muscles, frozen as a single block, were fixed in 4% paraformaldehyde and permeabilized using 0.2% Triton X-100 prior to staining with antibodies anti-Idh2 (HPand7831) and Idh3a (AV42237), both polyclonals from SIGMA.
Tissue blocks were fixed in 4% (w/v) buffered formaldehyde and embedded in paraffin.
Tissue blocks were fixed in 4% paraformaldehyde (PFA) in PBS overnight at 4°C and equilibrated in 30% sucrose in PBS overnight at 4°C.
Tissue blocks were fixed in 2.5% glutaraldehyde in 0.1 M phosphate buffered saline (PBS, pH 7.2).
The tumour blocks were fixed in 10% buffered formalin for 5 10 h and embedded in paraffin.
The tumor blocks were fixed in 10% buffered formaldehyde and embedded in paraffin.
The tissue blocks were fixed in formalin and embedded in paraffin.
Muscle tissue blocks were fixed in 2.5% glutaraldehyde in 0.1 M phosphate buffer (pH 7.4).
The tissue blocks were fixed in 10% neutral formalin and conventionally dehydrated.
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