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The quality of RNA extracted from tissues in one block was evaluated by the bioanalyzer (RIN = 6.7).
The level of sensory block was evaluated by the loss of pinprick sensation (20-gauge hypodermic needle).
Motor block was evaluated by modified Bromage scale (0 = no motion, 1 = finger movement, 2 = wrist flexion, and 3 = elbow flexion).
The patient was kept in the left side position for 15 min. The sensory block was evaluated by the pinprick test (22-gauge hypodermic needle), whereas motor block was evaluated by a modified Bromage scale (0 : no motor block; 1: hip block; 2: hip and knee block; 3 : hip, knee and ankle block).
As is typical in manual patch clamp, the onset of block was evaluated by repetitive stimulation at 0.1 Hz until a steady-state effect was observed, allowing cumulative use-dependent block, whereas in the IWB assay, stimulation was performed only once after exposure to the test compound for a fixed interval.
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Hematoxylin and eosin stained sections from each FFPE tissue block were evaluated by a pathologist at a GEICAM central laboratory.
Significance, including incompatible sites and recombination blocks, was evaluated by performing 1000 permutations using SNAP Map.
Flow of the fluid within the interstices among concrete blocks is evaluated by integrating the RANS equations.
Finally, syntenic blocks were evaluated by ColinearScan.
From the clinical tissue archive stored on site in the hospitals at University Health Network, a total of 50 FFPE blocks were evaluated by our breast pathologists (BY, NM, SD).
H&E-stained sections from paraffin-embedded tissue blocks were evaluated by a pathologist for tumor content and cellularity, and two cores from each block were selected for the TMA.
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