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The SPG block was applied using injections, bilaterally through the transnasal or palatal approach.
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Next, a block-matching algorithm is applied using the interpolated decoded frame.
The experimental conditions were applied using a block (Latin square) design (illustrated in Table 1), which provided efficiencies for treatment administration and calculation of condition order effects [ 18].
Restricted randomization with a 1 1 1 allocation ratio has been applied using randomly permuted block sizes.
Following rinsing in Tris-buffered saline Tween, endogenous peroxidase was blocked using Dako peroxidase block and a protein block was applied.
Endogenous peroxidase was blocked using Dako peroxidase block for 5 min and then Dako protein block was applied for 5 min after washing in tap water.
Primary antibodies were used according to the manufacturer's recommendations (1 : 100), and peroxidase block was applied for 10 min.
Within each group block randomization with variable block size (max. 6 per block) was applied.
Use of disteroids as larger building blocks was applied.
A randomized complete block design was applied with four replications using five treatments: 1) veticompost (composted poultry manure + vetiver grass prunes), 2) poultry tea (non-composted poultry slurry), 3) solid non-composted poultry manure, 4) poultry biochar and 5) an unamended control.
Subsequently, 5 % normal goat serum blocking buffer was applied.
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