Sentence examples for block was applied for from inspiring English sources

Serum block was applied for 30 min at room temperature before incubation of the primary antibody.

Primary antibodies were used according to the manufacturer's recommendations (1 : 100), and peroxidase block was applied for 10 min.

Endogenous peroxidase was blocked using Dako peroxidase block for 5 min and then Dako protein block was applied for 5 min after washing in tap water.

To investigate dose-dependency of the effects, four degrees of block were applied for each current.

Slides were placed into a wash buffer (Tris-buffered saline solution containing 0.05% Tween 20, pH 7.6 (DAKO)) and then a serum-free protein block was applied (DAKO) for 10 min.

Dako serum-free protein block was applied to the sections for 1 h, followed by incubation with the primary antibody (1 50 dilution) for 2 h.

Block was applied (5% NGS in PBS) for one hour before monoclonal anti-CD4-directly conjugated to Alexa-488 (1 100, eBioscience, UK) was added with 3% NGS in PBS for 2 hours.

After transfer and blocking with 5% skimmed milk in TBS, the primary antibody diluted in blocking solution was applied for 2 hours at 37°C.

In this study, multi-block analysis was applied for the first time to LIBS spectra provided by a portable LIBS system (IVEA Solution, France) equipped with three compact Czerny-Turner spectrometers covering the spectral ranges 200 397 nm, 398 571 nm and 572 1000 nm.

The primary antibody diluted in blocking solution was applied for one hour, except E-cadherin which was stained overnight at 4°C.

After three washes in PBS (5 min each), the secondary antibody, diluted in blocking solution, was applied for 1 h (Alexa 488, goat anti rabbit, 1∶1000, Invitrogen, Karlsruhe, Germany).