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The tissue blocks were transferred to a recipient "master" block using a Tissue Microarrayer.
Cores were placed on the recipient microarray block using a Tissue Microarrayer (Estigen OU, Tartu, Estonia).
Cores were precisely arrayed into a recipient paraffin block using a tissue microarrayer (TMA Grand Master, 3DHistech Ltd).
The tissue cores were precisely arrayed into a new paraffin block using a tissue microarray workstation (Beecher Instruments, Silver Spring, MD, USA), following methods described elsewhere.
Tissue biopsies of 0.6 mm diameter were taken from each donor block and mounted in a recipient block using a tissue array machine.
Tissue cylinders with a diameter of 0.6 mm were punched from selected areas of each 'donor' block and transferred to a recipient paraffin block using a tissue chip microarrayer (Beecher Instruments, Silver Spring, MD, USA).
Similar(34)
Tissue cylinders of 0.6 mm in diameter were punched from each paraffin block and transferred to the recipient paraffin block using a tissue-arraying instrument (Beecher Instruments Inc., Silver Spring, MD, USA).
The H&E slide was aligned with the corresponding "donor" block and a 0.6 mm cylindrical tissue specimen was taken from the selected area and transferred to a "recipient" paraffin block using a tissue-arraying instrument (Beecher Instruments, Sun Prairie, WI).
Parafinized tissue was arranged for cross and longitudinal sectioning and embedded in paraffin blocks using a tissue embedding station (Sakura Tissue-Tek TEC).
In order to exclude bias due to possible tumor heterogeneity, each patient had multiple tissue and tumor punches taken from formalin-fixed, paraffin-embedded blocks using a tissue cylinder with a diameter of 0.6 mm which were subsequently transferred into one recipient paraffin block using a homemade semiautomated tissue arrayer.
Tissue microarrays were constructed from formalin-fixed and paraffin-embedded archival tissue blocks using a tissue arrayer (Beecher Instruments, Silver Spring, MD) according to the previous description [ 29, 30].
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