From each donor block, three 0.6 mm diameter tissue cores were punched from tumor areas and transferred into a recipient paraffin block using a custom-made precision instrument.
Per patient, three tumour tissue cores of size 0.6 mm were transferred to a recipient paraffin block using a custom-made precision instrument.
Afterwards, the tissue cylinders were inserted into a new "recipient" paraffin block using a custom-built instrument (Beecher Instruments, Silver Spring, MD, USA).
From the representative region two core tissue biopsies (diameter, 0.6 mm; height, 3-4 mm) from the invading front were taken and precisely arrayed into a new "recipient" paraffin block using a custom-built instrument [ 15].
Tissue cylinders of diameter 0.6 mm were punched out from the selected areas for each corresponding paraffin block and mounted into a recipient block using a custom-made precision instrument (Beecher Instruments, Silver Spring, MD, USA).
From each donor breast tumour tissue block, three 0.6 mm tissue cores were punched from tumour areas and transferred into a recipient paraffin block using a custom-made precision instrument.
Tissue cylinders with a diameter of 0.6 mm were then punched from selected areas of the 'donor' block and mounted into a 'recipient' paraffin block using a custom-made precision instrument (Beecher Instruments, Silver Springs, MD, USA).
From each donor block, three 0.6 mm diameter tissue cores were punched from the identified tumour areas and transferred into a receiver paraffin block using a custom-made precision instrument.
Tissue cylinders of 1.5 mm diameter were punched from these areas and precisely arrayed into a new 'recipient' paraffin block using a custom-built instrument (Beecher Instruments, Silver Spring, MD).
A single biopsy (0.6 mm cores) from each tumour was taken from the area chosen by an uropathologist and placed in the paraffin block using a custom-made precision instrument (manuel tissue microarrayer 1, Beecher Instruments, Sun Prairie, WI, USA).
Tissue microarray construction was made by identifying the area of highest tumour grade on H&E-stained H&E-stainedlowed by punching out three tislidesylinders followed selected areas of the donor byock and then mounted into a recipunchingraffin blout using a custhreeade precision instissuet (Beecylindersruments, Silver Spring, MD, USA).
