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We have recently shown that silymarin and other synthetic antivirals block the fusion process [11], [30].
Recent data obtained with Dengue virus showed that carrageenan may interfere not only with adsorption of virus to cells but also block the fusion event leading to uncoating of the nucleocapsid [36].
Nevertheless, it can also block the fusion between autophagosomes and lysosomes, leading to accumulation of autophagosomes.
Manzamine A could block the fusion of autophagosome and lysosome and abrogate autophagosome turnover [ 13].
Bafilomycin (BAF) is widely used to block the fusion of autophagosomes with lysosomes, resulting in accumulation of autophagosomes in the cytoplasm.
Bafilomycin A1, a type of macrolide antibiotic derived from Streptomyces griseus, has been reported to block the fusion of autophagosomes with lysosomes in tumor cells.
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To enable visualization of Lc3-II accumulation in autophagosomes, we first blocked the fusion of autophagosomes with lysosomes by treating the larvae with 2.5 μM chloroquine40 for 14 hours (h) before analysis.
Intriguingly, PLP2-TM induces incomplete autophagy process by increasing the accumulation of autophagosomes but blocking the fusion of autophagosomes with lysosomes.
Chloroquine, as expected, induced autophagy but blocked the fusion autophagosomes-lysosome thus preventing the completion of the autophagic flux.
Bafilomycin A1 specifically inhibits AVO formation by blocking the fusion of autophagosomes and lysosomes (Yamamoto et al, 1998).
Vacuolin-1 treatment also blocked the fusion between endosomes and lysosomes, resulting in a defect in general endosomal-lysosomal degradation.
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