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Between each block subjects were given the opportunity to rest, after which they were presented with a screen directing them to check the alignment of the stimuli before commencing the next block.
At the end of the block subjects were given 3000 ms to respond by pressing a key marked "0", "1", or "2" using their right hand.
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Subjects were given blocks of 0-back, 1-back, 2-back, and 4-back, with half of the 2-back blocks occurring immediately after a 4-back block ("2-back/4"), and the remaining 2-back blocks occurring immediately after a control-condition 1-back block ("2-back/1").
All subjects were given modified twin block appliance with jack screw and labial bow incorporated in the maxillary plate.
Subjects were given propranolol, which blocks adrenaline receptors, before reading either an emotionally upsetting or closely matched but emotionally neutral story.
Subjects were given trials grouped in blocks, with 16 20 trials/block.
Subjects were given practice trials before each block and had the opportunity to take breaks.
Subjects were given time to rest between blocks.
Subjects were given a 30-s break between blocks; after 20 s of the break, the instruction screen appeared for 10 s to remind the subjects which letter corresponded to which trial type.
Prior to the scanning, subjects were given a practice version of the task with 2 bisection and 2 control blocks in order to familiarize them with the task.
Subjects were given visual feedback at the end of each fMRI run instructing them to respond more quickly in the next speed blocks if their mean RT (for all speed blocks within the run) was greater than 9 s or if their mean RT was less than 2 seconds faster than their mean RT for accuracy blocks.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com