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DNA-binding proteins bind to the duplex and block strand exchange, thereby suppressing the fluorescence elevation.
Several proteins, including histone octamer, p53, TRF1, and TRF2, have also been found to block strand exchange.
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Hybridization to a blocking strand denatured the DNAzyme.
VP-16 is the topoisomerase inhibitors, it can interact with the enzyme to stabilize topoisomerase-DNA complex, blocking strand-passing activity, thereby resulting in DNA breakage [ 25].
PARP inhibitors, which block single-strand break repair, increased the rate of nick-induced HR up to 1.7-fold but did not affect DSB-induced HR or mutNHEJ.
When minute agar blocks containing mycelium were submerged in distilled water, mycelial strands grew towards the water surface and eventually gave rise to floating fruit bodies connected to the parent agar block by strands of hyphae.
In contrast, blocking of strand exchange by a target protein results in lower fluorescence intensity.
To our knowledge, this is the first report to show that NF-κB blocks spontaneous strand exchange.
PARP inhibitors that block single-strand break repair (SSBR), a pathway related to BER, have shown clinical benefit in BRCA1/2 germ line-deficient breast and ovarian cancers.
As a first-generation integrase inhibitor, dolutegravir blocks the strand transfer step of integration of the viral cDNA into the host genome.
These complex structures, especially inverted repeats, might be blocks for strand synthesis during DNA replication, interfering with the activity of DNA polymerase.
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CEO of Professional Science Editing for Scientists @ prosciediting.com