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By using a 130 µm3 block of serial electron micrographs [29], where we explicitly reconstructed all dendrites, axons, glia and synapses, we show that high-end light microscopy is sufficient to study densely labeled populations of synapses in neuropil, as well as determine identities of the pre- and post-synaptic fluorophores tagging each individual synapse.
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The functions of the generating codes can be represented with polynomial equation or binary sequence as shown in Figures 4 and 5. Figure 3 Block diagram of serial concatenation convolutional SCCC encoder.
From each paraffin block of tumour, serial sections with a thickness of 5 μm were prepared and then stained with nuclear Fast Red (Sigma-Aldrich, St Louis, MO, USA).
Using the methods of serial block sections of the entire endometrium of the uterus surgically removed for smaller endometrial carcinomas, we found 131 "small endometrial carcinomas".
Movie S4. 3D Reconstruction of Serial Block Face Images Showing the Contact between a Migrating Schwann Cell and an Endothelial Cell Tubule, Related to Figure 4 3D-reconstruction showing direct contact between a migrating Schwann cell (green) and a tubule of HUVECs (red) within a fibrin gel as shown in Figure 4B.
Figure 11 shows a reconstructed volume comprised of serial block-face images collected with 3-keV electron-probe energy and 1.5-keV landing energy.
To further determine the relationship between axonal degeneration and mt fission in the glial lamina of glaucomatous D2 mice, we first assessed axonal degeneration by measuring the maximum intensity projection (MIP) of serial block-face scanning electron microscopy (SBEM) volume rendering and measured density, surface areas and volumes.
Figure 6 Block diagram for iterative decoder of serial concatenated code.
From each paraffin block of representative tumour areas, serial sections with a thickness of 10 μm were prepared and stained with nuclear Fast Red (Sigma-Aldrich, Tokyo, Japan).
Archived hematoxylin & eosin (H&E) stained slides were reevaluated by two experienced pathologists (PB, IK) for accuracy of diagnosis and formalin-fixed and paraffin-embedded tissue (FFPE) blocks were selected for generation of serial tissue slices for immunohistochemistry (IHC).
While the plates incubated with block solution, serial dilutions of the GST-FlpA, GST-FlpA-D1, GST-FlpA-D2 and GST-FlpA-D3 were made in PBS to produce concentrations that ranged from 1000 nM to 7.815 nM.
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