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A homology search against the PDB database resulted in no significant alignment without extensive gaps for this block of sequence.
Domains of unknown function (DUF) are often annotated in case a clear block of sequence similarity is found within a protein family with unknown function.
Sequence alignments of this region, using the five Mt genomes of Tetrahymena species showed a 94 bp conserved block of sequence in this variable intergenic region (Figure S2).
Interestingly, the more amino terminal SIM1 is in a very highly conserved block of sequence, but this almost completely lacks acidic residues (Figure 1C).
Let s min = 2 and check the sub-sequence 〈 CT GA〉 in the first block of sequence 1, its sup is 2, so it is a frequent pattern in the first block of sequence 1.
Interestingly, the second largest block of sequence identity encompasses the three yar promoters (Soshnev et al. 2008).
Similar(51)
Towards the telomere, this block of sequences is followed by other subtelomeric sequences duplicated on several chromosomes (Fig. S3).
We performed the same analysis in the Arabidopsis thaliana genome, and again, no syntenic block of sequences could be detected (Additional file 5).
Also the BAC 809J1 belonged to the 4q35 chromosome; in fact it aligned (> 98% of identity) with the block of sequences that in human chromosome 4q35.2 are comprised between DUX4c and the D4Z4 cluster (Table S4).
Overall, protein folds and gross functions can be readily transferred from one protein to another by transplanting large blocks of sequence (i.e., domain recombination).
The existence of blocks of sequence conservation may however not be necessary for the conservation of enhancer activity [7].
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com