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A blinded examiner counted the number of blinks per minute at baseline and before the last block of measurement (after the HFS intervention).
In each block, 15 responses per stimulation condition were recorded followed by a pause of 5 min between each block of measurement.
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The thresholds reported for each observer were based on four blocks of measurement, i.e. a total of 400 trials.
We hoped that the resulting complex target definition procedure will hide for the subjects the fact that had a relative probability of only 98.3% and was never one of the two targets across the multiple blocks of measurement.
Single pulse and paired-pulse stimulation was intermingled in a pseudo-randomized fashion during the blocks of measurements.
The shine-through paradigm is easily applicable because it can be applied with two blocks of measurements each lasting less than 5 min. VD estimation takes another 10 20 min. 6.
All these in vivo protocols in humans are inspired by former work about spike timing dependent plasticity on slice preparations [25] Before and after HFS, the excitability of the blink reflex circuit was assessed in blocks of measurements (Fig. 1 and 2).
The abovementioned blocks of measurements are presented schematically in Figure 1.
In the same way as the first experiment, blocks of measurements were recorded, now at a fixed frequency of 100 Hz.
For the first experiment (Fig. 1), five blocks of measurements took place for each TENS frequency, 5 min of registration without TENS, and followed by 5 min with TENS (so each TENS frequency had its own control-baseline).
As a component-based estimation method, the PLS-PM works as an iterative algorithm that separately analyses the blocks of the measurement model and estimates the path coefficients in the structural model (28).
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