Sentence examples for block buffer and incubated from inspiring English sources

Fluorescent secondary antibodies were diluted in block buffer and incubated for 30 minutes at room temperature.

Antibodies were diluted in block buffer and incubated overnight at 4°C.

Both primary and secondary antibodies were diluted in block buffer and incubated for one hour at room temperature followed by three washes with TBST buffer (25 mM Tris-HCl pH 8, 150 mM NaCl, 0.1% Triton X-100).

Monoclonal antibody was diluted 1 500 in block buffer and incubated on the blot at room temperature for 2 h.

Following the Click-it chemistry using the 594 fluorophore, slides were blocked for 1 hour in Cell Signaling block buffer, and incubated overnight with a rabbit anti-phospho histone H3 antibody (Cell Signaling Technology #9701).

Then, the cells were fixed with 3% PFA-PIPES and 3%PFA-Na2BO4PFA-Na2BO4d 10 min, respectively, permeandlized with 0.10 Triton-X 100 for 30 min, and blocked with 5% norespectivelyserum in permeabilizedn. Guinea pig sourced antibody against insulin (EuroProxima, The Netherlands) withdiluted in 5% block buffer and incubated overnight at 4 °C.

Cells were subsequently washed three times in blocking buffer and incubated with the appropriate secondary antibody diluted in blocking buffer (donkey anti-rabbit AlexaFluor 647 or donkey anti-mouse AlexaFluor 647 (1:1000; Jackson ImmunoResearch)) for 2 hours at room temperature.

Samples were permeabilized in 0.1% Triton-X in PBS for 15 minutes at room temperature, washed in blocking buffer, and incubated in 1 100 tubulin-FITC (Cell Signaling, Beverly, MA) and 1 50 rhodamine phalloidin (Life Technologies) overnight at 4 °C.

Anti-GPER (MBL, Woburn, MA) was diluted 1∶250 in blocking buffer and incubated overnight at 4°C.

All primary antibodies were diluted in blocking buffer and incubated at 4°C for 18 h.

Primary antibodies were diluted in the blocking buffer and incubated with cells for 45 min at RT.