Sentence examples for block buffer from inspiring English sources

Non-specific binding sites were blocked with block buffer (100 ml Tris-buffered saline supplemented with 1 mM CaCl2, 0.5 mM MgCl2, 10% Normal Goat Serum (Harlan Lab Sera) and 1% Teleostean Gelatin [Sigma]) for 0.5 h.

Each well was washed with 3 × 200 μL of phosphate-buffered saline (PBS) and blocked with block buffer (Pierce, Rockford, IL).

It segments every incoming packet and keeps blocks in the block buffer.

Then, these IW blocks are stored in a local block buffer.

These are then fed to the active transport block buffer along with those that are to be retransmitted.

The case study is setup with (k=7) locations (Response area, five x 1 block buffer areas, and a control area).

The case study is setup with (k=5) locations (Response area, (3 times 1) block buffer areas, and a control area).

8 ml biotin (Vector Labs) was added to every 50 ml of "5/5" block buffer.

Antibodies were diluted in block buffer and incubated overnight at 4°C.

Fluorescent secondary antibodies were diluted in block buffer and incubated for 30 minutes at room temperature.

Culture supernatants were serially diluted in block buffer and added for 2 4 h to allow Env gp140 capture and incubated with a 1∶1000 dilution of pooled HIV-1 positive patient sera in block buffer.