Sentence examples for block at room from inspiring English sources

Dissolution of polystyrene-block-poly ethylene-co-propylene) (polystyrene-block-poly ethylene-co-propylenehe polystyrene-block-poly ethylene-co-propylenefor the polystyrene-block-poly ethylene-co-propylenetion of metastable micellar structures, which convert into stable micelles with molar mass reduced several times after heating above ∼50°C.

Cells were then pretreated with DakoR Protein Block at room temperature (RT) for 1 h, followed by incubation with primary antibodies (Runx2, PPARγ, Sox9, and MyoD; 1∶50; Santa Cruz Biotechnology) at 4°C overnight.

The plate was washed with 100  μL PBS, and 2% (w/v) BSA in PBS was added to block at room temperature for 1.5 h.

The membranes were blocked at room temperature in 5% milk/Tris-buffered saline Tween 20 solution.

Embryos were washed 10 times in PBST (2 hrs total) at room temperature and blocked at room temperature for a further 1 hour.

After electrophoresis, proteins were transferred to PVDF membranes (Bio-Rad) and blocked at room temperature with 2% Enhanced Chemiluminescence (ECL) Advance Blocking Agent (Amersham, GE Healthcare) in TBS-T.

After hybridization, the hybridization cartridge was disassembled and the beadchip slide array was placed in a 55°C high temperature wash followed by washing and blocking at room temperature.

The membrane was blocked at room temperature for 1 hour in 5% skim milk diluted in PBS-Tween 20 (0.5% v/v).

After fixation, samples were washed in the same solution without fixative and blocked at room temperature with 10% bovine serum albumin.

Membranes were stripped of antibodies for 30 min (Re-blot plus solution; Millipore), rinsed, and blocked at room temperature for 2 h.

Cells, grown on glass coverslips slides in 24 mm Petri dishes, were fixed in 100% methanol at –20°C for 7 minutes, washed with PBS and then blocked at room temperature for 1 hour in 3% BSA/0.1% (v/v) Triton X-100/PBS.