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Block age, RNA extraction method and optimal concentration for a successful DASL assay, were previously evaluated [8], [10], [16], [37] and are aspects beyond the scope of this study.
For example, tissue block age, a multi-parameter involving at least storage conditions and continuous degradation of nucleic acids after embedding [13], [41], was vaguely related to the degree of DNA fragmentation in our series.
If comparing all samples, the efficiency of the fragmentation test was related to the A260/280 ratios and to block age (Mann-Whitney p = 0.003 and p = 0.010, respectively), in line with previous observations for continuing nucleic acid degradation after fixed tissue embedding [41].
In the final block, age, gender, SES, years of education, and marital status were added.
Amplification was not influenced by tissue type or paraffin block age.
Therefore, we examined whether FFPE block age at RNA extraction contributed to array performance.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com