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The lower surfaces (with migrant cells) were captured by photomicroscopy (BX51 Olympus, Japan), and the cells were blindly counted (five fields per chamber).
Mesenchymal cells were blindly counted by different laboratory members to prevent any bias.
To quantify the expression, spots and cells were independently and blindly counted twice, in subzones of the tumour.
The number of caspase 3-positive cells was blindly counted by a researcher blinded to the assignment of experimental groups.
Approximately, 500 nuclei were blindly counted for each brain, by two independent observers, on 14 samples (5 non-diseased, 9 diseased).
Eyes were collected at P21. GFP+ rod photoreceptors (in the ONL), bipolar cells (Otx2+ in the INL) and Müller glia (Otx2− in the INL) were blindly counted from random images of the central retina.
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Dissect lungs and blindly count the number of macrometastatic nodules on every side of all separated lobes of the lung using a stereomicroscope.
In order to find whether the ApoE COG 133 peptide restores epithelial cell renewal in ApoE knock-out mice, the mitotic index was also calculated in these mouse strains by blindly counting well-defined mitotic figures at the crypt proliferative compartment, as described elsewhere [ 6].
Blindly count tumors per mouse and measure tumor diameter macroscopically.
The counts were scored blindly and counted in duplicate slides.
Images of the lower surfaces (with migrant cells) were captured by a photomicroscope (5 fields per chamber) (BX51 Olympus, Japan), and the cells were counted blindly.
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