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Fixed kidney tissue was stained with hematoxylin and eosin for evaluation of glomerulosclerosis and tubular damage and cryopreserved sections of kidney tissue were stained with FITC conjugated antibodies to IgG, IgM, and C3 for assessment of immune complex deposition that was scored by a blinded reader using a scoring system previously established in our laboratory[30].
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Radiographs of the hands and feet were obtained at baseline and week 24, and were read by two blinded readers using a modified version of the total Sharp score (mTSS) that included assessments of distal interphalangeal joints [ 13].
Radiographs of hands and feet (at baseline and 18 months) were assessed in chronological order by two blinded, trained readers, using the modified Sharp-van der Heijde (SvdH) score (range 0-448; higher scores indicate more joint damage).
Two independent graders scored all histological sections in a blinded manner using a modified Mankin Histology Scoring System [23].
Histological assessment was performed by two blinded investigators using a score as described previously [21].
Two independent, blinded investigators counted the cells using a microscope.
Lastly, absorbance was measured at 490 nm using a microplate reader (Bio-Tek Synereader micreadere reader).
Absorbance at 540 nm was measured using a microplate reader (iMark microplate reader; Bio-Rad).
Luminescence was recorded using a plate reader.
using a plate reader (Thermo Fisher Scientific Inc., MA, United States).
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