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Two independent graders scored all histological sections in a blinded manner using a modified Mankin Histology Scoring System [23].
Using this eight-gene predictor set, classification of a sample as being either PC or a healthy control was attempted in a blinded manner using a sample set consisting only of the samples that were not used to create the predictor set (i.e. 24/68).
Light microscopic studies were reviewed in a blinded manner using a histopathological scale previously described [ 20].
Two 5 μm slices from the specimens of the pancreas were examined in a blinded manner using a light microscope.
For the ethics procedure, this work was conducted in a blinded manner using a linkable anonymizing method.
Evaluation of arthritis in ankle joints was peformed in a blinded manner using a semiquantitative AS ranging from 0 to 3 as described previously [ 10].
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Histological assessment was performed in a blinded manner, using an established scoring system for synovial hyperplasia (from 0 = no hyperplasia to 3 = most severe hyperplasia) and inflammatory cell infiltration in the synovium (from 0 = no inflammation to 3 = most severe inflammation).
The risk of bias in the studies was assessed by a single investigator (ALW) in an un-blinded manner using a tool developed by the Cochrane Collaboration (focusing on specific domains including sequence generation, allocation concealment, blinding, incomplete outcome data and selective outcome reporting) [11].
Metastatic foci were visualised in a double-blinded manner using a light microscope.
Following informed consent patients were randomised in a double-blinded manner using a Latin Square fashion to one of two open parallel groups.
The patients received allocations in a randomized, double-blinded manner using a computer-generated distribution (QuickCalcs, GraphPad Inc., La Jolla, CA, USA).
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