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The remaining high-quality videos were analysed (1) by an experienced user blinded for the study protocol using a manual analysis software (AVA software version 3.2, Microvision Medical, Amsterdam, The Netherlands) and (2) a software including an automatic video analysis of microcirculatory videos (AVA software version 4.2, Microvision Medical, Amsterdam, The Netherlands).
The investigators were blinded for the study treatment and the block size.
12 13 One reviewer, who was kept blinded for the study outcome to ensure objectivity, reviewed all histopathological reports according to a predefined protocol to ensure uniformity.
An independent Safety Monitoring Board (SMB), blinded for the study groups, will be installed to review complications related to fertility treatments, pregnancy, delivery and neonatal outcome.
18 The radiation oncologists and radiation technicians were blinded for the study aim and procedure at the time of treatment of all patients.
Echocardiographic evaluation was performed 1 day before cardiac catheterisation by GE Vivid 7 and 9 ultrasound system by an investigator blinded for the study objectives.
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The subjects will be randomly allocated into either of the two arms of the study by an external statistician blinded for the intervention and study participants and will only be provided with a randomisation number for each participant and severity of OA in knee according to x-ray classification.
Patients are blinded for this study.
The staff that performed the physical examination in the intervention region was not blinded for the goal of the study, but they were unaware of the values of the pre-intervention measurement when conducting the post-intervention measurement.
Operation time per animal did not exceed 15 min and operators (PK, CK and MA) were blinded for the respective genotypes throughout the study.
Patients are blinded for the treatment allocation since the study intervention will be done under general anaesthesia.
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