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Initially, all workers were blind to sample origin (i.e. habitat).
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As our analysis software was unable to distinguish between lipofuscin and the autofluorescent aggregates, a researcher blinded to sample origin separated each autofluorescent item identified by the software into distinct categories based on their distinctive morphology: large, dense autofluorescent aggregates or the more granular lipofuscin (Figs. 7a, S8).
Genotyping was blind to sample outcomes.
Genotypes were called in duplicate blind to sample identity and blind to the other rater.
blinded to the sample origin, and an average of the replicates was determined after unblinding.
All laboratory investigations were performed by the investigator blinded to the sample origin.
Quantification of pathology was performed on all cases by a researcher blinded to the sample origin.
Histological analysis of the repaired tissue was performed for each specimen by observers who were blinded to the sample origin.
Investigators were blinded to sample group membership and specific diagnoses.
CNAs were analyzed blinded to sample histology.
Investigators were blinded to sample identity.
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