Exact(2)
Blind quantification of immunocolocalization degree by Mander's coefficient analysis revealed an age-dependent reduction of the fraction of RyR overlapping with VDAC (m1) without significant differences in either RyR or VDAC total fluorescences.
Blind quantification of the injected and uninjected contralateral retina, showed reduced neovascularisation after 1 or 10 ng VEGF121b (see Figure 6B-C, uninjected control vs 1 or 10 ng VEGF121b P<0.05 or control injection vs 1 or 10 ng VEGF121b P<0.05).
Similar(58)
Network branch number counting was performed by a single investigator who was not the person who captured the images, thus ensuring a double-blind quantification method.
In order to clarify that the visible pores are poly-Perforin-2, we have added blinded quantification of pores present with different conditions.
Panels E and G are close-up images of the boxed region in C and F. Blinded quantification of pore amount with different conditions is demonstrated in Figure 6 figure supplement 1. DOI: http://dx.doi.org/10.7554/eLife.06508.020 10.7554/eLiFigure08.021 Figure 5 figure supplement 1.
All samples were blinded for quantification.
The investigator was blinded during quantification.
EM images were blinded before quantification and scored independently by two investigators.
The experimenter was blinded during quantifications.
Three-dimensional images were stacked for dopamine neuron cluster identification and quantification, blind to the genotypes.
For each experiment unless otherwise stated, a minimum of four wells from at least three separate cultures (derived from different animals), were used for quantification, blinded to conditions.
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