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The blind passages make it likely that sperm will be ejaculated into the wrong place and thus fail to fertilise the duck's eggs.
We were not able to detect the virus replication, neither by plaque assay on BHK-21 cells nor in blind passages in BHK-21 cells, (data not shown).
Modified Vaccinia virus Ankara (MVA) is a replication-deficient attenuated poxvirus that was derived from Vaccinia virus by Mayr et al. through more than 500 blind passages in chick embryo fibroblast (CEF) cell culture [1].
FMDV was considered extinct when no virus infectivity and no viral RNA that could be amplified by a highly sensitive RT-PCR protocol, could be demonstrated neither in the supernatant of the passage that harbors the putatively extinguished virus, nor after 3 blind passages in BHK-21 cells, in the absence of any drug.
To determine if virus was produced even though no virus was detected by plaque assay on BKH-21 cells, supernatants of D5 CD172+ cells infected with FMDV were harvested at different time points (6, 24 and 48 hpi) and were subjected to two blind passages in BHK-21 cells.
Three blind passages were performed on negative samples.
Similar(17)
Previously, by blind passage, a vvIBDV Gx strain was attenuated to the Gt strain, and a strain CEF-9 with intermediate characters was obtained during attenuation.
However, the traditional method of vaccine development with blind passage could not fit the style of the emergency prevention of IBDV.
Limitations and complications of the ILMA may include aspiration, esophageal intubation, damage to the larynx or other tissues during blind passage of a tracheal tube, and edema of the epiglottis.
Our prototype RepliVAX JE replicated poorly in cells, but blind passage produced a better-growing derivative, and analyses of this derivative allowed us to engineer a second-generation RepliVAX (RepliVAX JE.2) that grew to high titers.
Irradiated specimens were tested for infectivity by inoculation onto Vero E6 cells, and examined for cytopathogenic effects for 10 days, followed by blind passage of the cells and testing for the growth of SARS-CoV by PCR [29].
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