Exact(17)
To validate the automated nuclear shape analysis, we also assessed nuclear morphology using the standard technique: manual blind counting.
To judge the impact of rapamycin and RAD001, we first scored the percentage of nuclei with abnormal morphology in the usual way by manual blind counting.
The investigators conducted blind counting for each quantification-related study.
The investigators conducted blind counting for all quantification.
The numbers of TUNEL-positive or BrdU-positive beta-cells were determined by blind counting with a fluorescent microscope.
This approximation requires, besides confocal microscopy, the participation of a qualified and blind counting supervisor to avoid subjective appreciations of the imaging interpretation of the data.
Similar(43)
Sister chromatid exchanges (SCEs), chromosomal aberrations (CAs), and micronuclei (MN) (Table 1) in peripheral blood were blind counted (Xie et al. 1999).
At least 500 cells per slide and three slides per condition were double-blind counted.
Two people completed triple blind counts of each of the specimens and then the average of these counts was used as the age of the animal.
The traditional method of measuring blebbing is a manual, blind count of the percentage of blebbed nuclei.
For quantification of enlarged endosomes in patient fibroblasts blind counts were performed on 600 cells per line across three independent experiments using cells matched for passage number.
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