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The blend was immediately transferred into a graduated cylinder.
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The supernatant was immediately blended with Ni-NTA resin (Novagen), pre-equilibrated with the binding buffer, and agitated at 4 °C for 1 h.
In our system, the aqueous silk solution and HAp NPs colloidal suspension combine together at the center of the three-way connector for a short time without giving enough time to precipitate, and this blend solution is immediately ejected out to form nanofibers. Different weight ratios of 10%, 30%, and 50% of HAp NPs were used as blend solution to electrospun nanofibers.
He was immediately hooked.
And was immediately sorry.
He was immediately popular.
I was immediately hooked.
Walker was immediately intrigued.
Power was immediately released.
That was immediately granted.
She was immediately popular.
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