Sentence examples for blend of oligo from inspiring English sources
Human RNA (500 ng) was reverse transcribed with the QuantiTect RT kit (Qiagen), containing a blend of oligo dT and random primers.
Total RNA was extracted by hexadecyl-trimethylammonium bromide (CTAB) and purified as previously described [ 42]. cDNA was synthesized from the total RNA samples using a blend of oligo (dT) and random primers and an iScript™ cDNA Synthesis Kit (Bio-Rad, Hercules, CA, USA).
cDNA was synthesized from the total RNA extracted from different individuals at different times using the Qiagen Quantitect RT kit which uses an optimized blend of oligo-dT and random primers to promote high cDNA yields, even from 5′ regions.
RNA from the same animal and tissue samples were used to validate the microarray data. 1 μg of total RNA from affected and control samples was reverse transcribed into cDNA using a blend of oligo-dT and random hexanucleotides and the QuantiscriptReverse Transcriptase (Quiagen).
cDNA was generated with a blend of random hexamers and oligo-dT 1 : 1 (ABgene, Surrey, UK) and the target cDNA sequences were amplified by quantitative PCR in a fluorescent temperature cycler (Mx 3000P Real Time PCR System, Stratagene).
Affy_oligo id indicates the name of oligo sets in Affymetrix array.
NSF_Oligo id indicates the name of oligo sets in NSF45K array.
Each well contained 1 nmol of oligo.
It's a nice blend of both".
A blend of aglianico and piedirosso.
