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Serum from tail bleed samples was used to measure IFNγ and TNF-α by ELISA (R&D Systems, UK).
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At the end of the boosting period a terminal bleed was conducted and serum samples were used to purify IgG antibodies as described below.
Two-tailed t-tests for independent samples were used to compare cumulative PCA, bleeding time, total blood loss, and graft size across treatment groups.
Two training samples are used.
When random samples are used, there is always potential error that comes with using a sample.
DMEM sample was used as reference sample.
As only one sample was collected at baseline and final bleeds (i.e. weeks 0 and 69), only prevalence data from the first sample collected at double bleed time points was used for the analyses of time trends across the entire time of follow-up.
The final bleed was used for affinity purification.
Antibody from the third bleed was used for all experiments.
A purposive sampling strategy was used in order to include patients with different underlying conditions including, AUB, post partum bleeding and GI disorders.
Electronic compensation was used to eliminate bleed through fluorescence.
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