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Because these non-ratiometric measurements were affected by bleaching of the indicator dye (Fig. 3B), the data was corrected by subtracting the bleaching contribution estimated by the slope difference (a0) from first and last exposures to control saline, quantifying the effect of tested compounds as: [ a1−a0)/(a2−a0) ×100].
The signal drift of approx. 10% in the absence and 1.1% in the presence of carbon dioxide over 7.5 h is attributed to the bleaching of the indicator dye.
Similar(58)
This approach offers additional advantages; due to the excitation by energy transfer, photo bleaching of the pH indicator can be minimized.
While this allows us to gain important insights into how cortical neurons represent sensory information, factors such as photo-bleaching of the Ca2+ indicator limit imaging duration (and thus the numbers of stimuli that can be tested), which in turn hampers the full characterization of neuronal response properties.
Since both sensor calibrations drop the same amount it is assumed, that the sensor itself is not prone to photo bleaching but may suffer from leaching or migration problems due to incomplete encapsulation of the indicator and reference dyes.
"All of the indicators were there.
Regular bleaching of clothes weakens the fiber.
Bleaching was also evaluated qualitatively by observing the horizontal and vertical bleaching of HA agar around the inocula.
The indicator has limits, of course.
Bleaching of immobile fractions or cell motion increases the variance.
Bleaching of fluorophores was negligible.
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