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To analyze the expression of the silkworm apoptosis-related genes in different developmental stages according the chips, a BlastN alignment was performed using the silkworm different developmental stage database.
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The alignment was performed by BLASTn (Altschul et al. 1990) with the following stringent parameters: '-perc_identity 95 -evalue 1e-30 -word_size 50'.
Alignment was performed as previously described [16].
Sequence alignment was performed using Jalview as alignment editor [37].
Alignment was performed using ClustalX.
Reciprocal BLAST [ 25] (v.2.2.22 with options -a 8 -F F -p blastn -m 7) alignments are performed between the contig and HLA CDS or genomic sequence databases depending on the read source (RNA-Seq or WGS and exon capture), parsed at runtime using PERL Bio::SearchIO modules and summarized.
Local alignments were performed using BLASTn [ 65] and visualised with the Artemis Comparison Tool (ACT) [ 69].
Sequence alignments were performed using blastn and bl2seq from BLAST+ suite [ 78].
Sequence alignments were performed using BLASTn (default parameters and a maximal e-value of 10-5) and only the best homologies were considered further.
ClustalW multiple-sequence alignments were performed for each blastn search.
Validation of the tBLASTx and BLASTn assignment was performed for 50 S.
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