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BLAST matches can be filtered according to a minimum percentage identity or E-value cut-off (or indeed any available BLAST option).
To select these additional species, we (1) selected taxa which displayed a close relationship with the species studied according to previous work (see references and additional details in supplementary materials) and (2) using the BLAST option with all the previously selected sequences of the species studied.
Sequences containing possible frameshifts were identified with the ' w 20' BLAST option and disabled low complexity filters.
The BLAST option in the home page menu (1 in Figure 5) allows the user to BLAST sequences of interest against the 90, 98, and 90e databases (1.2 in Figure 5).
Finally, in order to identify possible events of horizontal transfer, consensus sequences from each family were submitted to the Blast option on the NCBI Web site and a multitude of sequenced organisms were screened.
Further analyses using the NCBI BLAST option to align two sequences and the CHAOS/DIALIGN software detected an overlapping region between the Chinese Merino BACs (GenBank: FJ985874 with GenBank: FJ985856) that was not reported by Geneious.
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At present, some of the Blast options we used are no longer available.
The user may provide the advanced blast options in order to narrow down the search results.
A liberal e-value [ 23] cut-off of 1 was employed and the -v and -b BLAST options were set to 1.
With this interface, data can be queried using different strategies, such as several Blast options or direct word search for annotation or GO terms.
Mcm8 and Mcm9 homologues were checked by using both BLASTp and tBLASTn searches in BLAST options of the respective genome projects (listed in Additional file 6), with putative Mcm8 and Mcm9 protein sequences of closely related taxa as a BLAST seed for these sub-analyses.
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