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All BLAST hits with a BLAST e-value ≤1 were considered repeats.
Orthologs are usually identified from bidirectional best BLAST hits, which requires doing all-versus-all BLAST.
Assessment of the top BLAST hits' species distribution for S. sulcatum is shown in Fig. 2.
b E-value distribution of BLAST hits for each unique sequence against the Nr database.
c Similarity distribution of the top BLAST hits for each sequence against the Nr database.
Other BLAST hits were discarded.
50 top blast hits were retrieved and used for annotation.
Sequences without significant blast hits were defined as Waco9-specific.
Each protein is grouped with all its BLAST hits and then iteratively grouped with their BLAST hits until no more sequences are added to the UPC.
Identical top BLAST hits suggested the genes were putative orthologs; non-identical top BLAST hits suggested the possible presence of paralogs, which were excluded from the analysis.
Although the best BLAST hits resulted in 3 CRNs, the 16 characterized CRNs [7], [19] were found within the high-scoring segment pairs (HSPs) of these BLAST hits.
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