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10 sample blanks were also prepared following the same procedure.
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A reagent blank was also prepared for the Ni II) solution.
For each digestion, a blank was also prepared with the same amount of acids without soil sample.
Blank NPs were also prepared by the same method without adding gemcitabine at any stage of the preparation.
Quality control (QC) samples at four different concentrations: 0.45, 1.28, 42.50 and 425 ng/mL in blank rat plasma were also prepared in a similar manner and treated as LOQ QC, LQC, MQC and HQC respectively.
Blank (without drug) formulations were also prepared for formulations F1 F12.
The blank and standard samples were also prepared in a similar manner.
Blanks (acid mixture without sample) were also prepared and 'digested' in parallel.
In addition to control samples (DNA+EtBr), test samples (DNA+EtBr+test compounds), blank 1 (EtBr only), blank 2 (DNA only), wells containing DNA and compound were also prepared to test any change in the background fluorescence readings.
Blank and ROX loaded PLGA (ROX-PLGA) NPs were also prepared.
Blank wells coated with carbonate buffer without antigen were also prepared to test non-specific binding of the samples.
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