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Program IV: Triple trio from "Gallery," jump dance from "Totem," "Kites" from "Imago," shadow opening from "Totem," "Celebrants" from "Totem," "Blank on Blank," water study from "Sanctum". 44 minutes.
Results show that the proposed prototype can discriminate the samples measured (Blank water, acetone, toluene, ammonia, formaldehyde, hydrogen peroxide, ethanol, benzene, dichloromethane, acetic acid, xylene and dimethylacetamide) with a 94% classification success rate.
There was no detectable dufulin residue in blank water samples.
The constant blank (water) has been subtracted from all calculated values.
Blank water samples in different pH conditions: (A) pH=5; (B) pH=7; (C) pH=9; fortified water samples with rac-dufulin (spiked level 3.00 μg/mL) in different pH conditions: (D) pH=5; (E) pH=7; (F) pH=9.
The potassium acid phthalate solution (0.1 mol/L) was adjusted to pH 5, the potassium dihydrogen phosphate solution (0.1 mol/L) was adjusted to pH 7, and the boric acid/potassium chloride solution (0.1 mol/L) was adjusted to pH 9. The blank water samples showed undetectable levels of dufulin (Figures 2A, 2B, and 2C).
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The maximum absorbance (360 390 nm) of the supernatant was read against appropriate blanks (water, 2 mol/L HCl) and the carbonyl content was calculated using the molar absorption coefficient of 22 000 mol/L−1 cm−1 [22].
Template blanks (water) and extraction controls, consisting of lysis buffer minus tissue extract, were included in every assay.
All samples, blanks (water and acid matrix), and NIST standard reference material Oyster Tissue 1566 b were analyzed by the following method using Optima Grade Fisher Scientific hydrochloric and nitric acids that were certified to contain less than 0.0001 microgram (μg) mercury per gram (g) reagent.
Concentration 0.5 ml 1 N HCl was added to the sample and to the blank (distilled water).
For blank distilled water was used [ 25].
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